Hormonal
Perilipin phosphorylation by PKA is not required for Hormone-sensitive Lipase (HSL) translocation to lipid droplets, but is strictly required for the lipolytic activity of HSL once it is associated with the lipid droplet.
This research clarifies that the body's fat-burning machinery (HSL) can physically reach fat stores without the specific hormonal 'permission' (phosphorylation) previously thought necessary. However, without that hormonal signal, the fat is not actually broken down. This implies that while the machinery is present, the actual release of fatty acids depends on the hormonal state (e.g., stress, fasting, exercise) triggering the final catalytic step, not just the movement of the enzyme.
Thus, PKA-induced HSL translocation was independent of perilipin phosphorylation. However, Peri A-1-6 failed to enhance PKA-stimulated lipolysis... Thus, the lipolytic action(s) of HSL at the LD surface requires PKA-dependent perilipin phosphorylation.
Why this rating
High-quality in vitro and transgenic mouse models with rigorous controls (shRNA, mutant proteins), though not a human clinical trial.
Source
Perilipin Promotes Hormone-sensitive Lipase-mediated Adipocyte Lipolysis via Phosphorylation-dependent and -independent Mechanisms
Hideaki Miyoshi et al. · Journal of Biological Chemistry · 2006
DOI 10.1074/jbc.m601097200
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