Hormonal
Ingestion of an isonitrogenous non-essential amino acid (NEAA) formulation does not stimulate muscle protein synthesis (MPS) or activate mTOR signaling pathways in skeletal muscle cells, making it an effective non-bioactive control for protein bioactivity studies.
If you are looking to stimulate muscle growth, consuming non-essential amino acids (NEAA) alone will not trigger the necessary biological signals. Muscle protein synthesis is driven by essential amino acids (EAAs), specifically leucine. Use NEAA as a control or filler in studies, but rely on complete proteins or EAAs for actual muscle building effects.
Media conditioned by NEAA fed serum had no effect on protein signalling or MPS compared to fasted... In summary, media conditioned by NEAA fed serum did not result in activation of MPS.
Why this rating
The study uses an ex vivo human serum model with C2C12 cells; while rigorous for mechanism, it is not a direct human clinical outcome study.
Source
A cell-based evaluation of a non-essential amino acid formulation as a non-bioactive control for activation and stimulation of muscle protein synthesis using <i>ex vivo</i> human serum
Bijal Patel et al. · bioRxiv (Cold Spring Harbor Laboratory) · 2019
DOI 10.1101/713768
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