Hormonal
High-sensitivity ELISA assays are required to detect exercise-induced reductions in fasting insulin, whereas standard automated immunoassays (e.g., Immulite 2000) fail to capture these changes despite high correlation with international standards.
If you are tracking insulin response to lifestyle changes (like exercise or diet), ensure your laboratory uses a high-sensitivity ELISA assay. Standard automated immunoassays may report 'normal' or unchanged insulin levels even when significant metabolic improvements have occurred, leading to a false conclusion that the intervention is ineffective.
Critically, insulin measured using the high-sensitivity ELISA was able to detect a group mean reduction following exercise training, while the Immulite 2000 immune-assay could not (Figure 1).
Why this rating
The study uses three independent cohorts (n=444 total) and rigorous cross-validation, though it is a preprint.
Source
Biomarkers of Insulin Resistance and Their Performance as Predictors of Treatment Response in Overweight Adults
Robert J. Brogan et al. · The Journal of Clinical Endocrinology & Metabolism · 2025
DOI 10.1210/clinem/dgaf285
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