Research

Hormonal

Using assumed or estimated menstrual cycle phases (e.g., calendar-based counting) instead of direct biochemical verification (e.g., LH surge, progesterone levels) produces invalid and unreliable data in sport-related research.

Do not use calendar-based methods or apps to assign menstrual cycle phases in research or applied practice without biochemical verification. To accurately determine phases (e.g., follicular vs. luteal), you must measure biological markers such as urinary luteinizing hormone (LH) surges and serum/saliva progesterone levels. Relying on calendar counting alone is scientifically invalid due to high inter- and intra-individual variability in ovulation timing and the prevalence of subtle menstrual disturbances.

StrongRefutesVERY_HIGH confidence
Using assumed or estimated phases, however, amounts to guessing the occurrence and timing of ovarian hormone fluctuations and risks potentially significant implications for female athlete health, training, performance, injury, etc., as well as resource deployment.
Kirsty J. Elliott‐Sale et al. · Sports Medicine · 2025

Why this rating

This is a consensus opinion/editorial by a large interdisciplinary group of experts citing multiple studies showing discrepancies between assumed and verified phases.

Source

Why We Must Stop Assuming and Estimating Menstrual Cycle Phases in Laboratory and Field-Based Sport Related Research

Kirsty J. Elliott‐Sale et al. · Sports Medicine · 2025

DOI 10.1007/s40279-025-02189-3

narrative_reviewCited 42×
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DOI resolved against Crossref · corpus check 2026-06-10

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