Mixed
In vitro digestion using the INFOGEST protocol demonstrates that intact proteins from diverse sources (whey, collagen, zein, legumes, cereals) are fully hydrolyzed during the intestinal phase, although digestion-resistant peptides persist in all substrates.
When you eat protein from any source (meat, dairy, beans, grains), your digestive system breaks down the large protein structures into smaller peptides during the intestinal phase. While some resistant peptides remain, the large intact proteins are gone. This suggests that digestive capability is not the primary limiting factor for plant vs. animal protein absorption in a healthy gut.
In all substrates, no intact protein from the substrates was visually detected by SDS-PAGE after the intestinal phase of in vitro digestion with the INFOGEST protocol. However, digestion-resistant peptides were detected in all substrates after the intestinal digestion phase.
Why this rating
High-quality in vitro methodology (INFOGEST harmonized protocol) with robust analytical validation (SDS-PAGE, HPLC-MS/MS), though it is an in vitro model and not a direct human in vivo trial.
Source
Protein digestion of different protein sources using the INFOGEST static digestion model
Raquel Sousa et al. · Food Research International · 2020
DOI 10.1016/j.foodres.2020.108996
Related findings · Mixed
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