Research
Hormonal
High-sensitivity ELISA assays are required to detect changes in fasting insulin following lifestyle interventions, as standard automated immunoassays (e.g., Immulite 2000) fail to capture these treatment-induced improvements.
If you are tracking insulin levels to monitor the effectiveness of diet or exercise changes, ensure your lab uses a high-sensitivity ELISA assay. Standard automated immunoassays may report normal or unchanged levels even if your insulin has significantly improved, leading you to believe your lifestyle changes are ineffective when they are not.
GoodSupportsHIGH confidence
Critically, insulin measured using the high-sensitivity ELISA was able to detect a group mean reduction following exercise training, while the Immulite 2000 immune-assay could not (Figure 1).
Source
Biomarkers of insulin resistance and their performance as predictors of treatment response in overweight adults
Rebecca S. Brogan et al. · medRxiv · 2024
DOI 10.1101/2024.10.06.24314962
preprint · n=444Cited 2×
Read the paper DOI resolved against Crossref · corpus check 2026-06-10
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