Research
Hormonal
Serum from humans undergoing Continuous Calorie Restriction (CR) increases Sirt1 protein and PGC-1a mRNA levels in vitro, indicating metabolic and mitochondrial adaptations associated with longevity.
Continuous Calorie Restriction triggers cellular changes that support mitochondrial health and stress resistance. This suggests CR is effective for promoting longevity pathways, even if it doesn't always reduce proliferation as dramatically as ADF.
ModerateSupportsMEDIUM confidence
Cells cultured in serum from CR participants induced an increase in Sirt1 protein levels by 17% and a 30% increase in PGC-1a mRNA levels.
Why this rating
In vitro study using human serum; strong mechanistic signal but lacks in vivo longevity endpoints.
Source
In Vitro Cellular Adaptations of Indicators of Longevity in Response to Treatment with Serum Collected from Humans on Calorie Restricted Diets
Joanne Allard et al. · PLoS ONE · 2008
DOI 10.1371/journal.pone.0003211
mechanism_only · n=42Cited 70×
Read the paper DOI resolved against Crossref · corpus check 2026-06-10
More from this paper
- Serum from humans undergoing Alternate Day Fasting (ADF) induces in vitro cellular adaptations associated with longevity, including increased Sirt1 protein, reduced proliferation, and enhanced heat stress resistance.Moderate
- Alternate Day Fasting (ADF) induces greater stress resistance and Sirt1 upregulation in vitro than Continuous Calorie Restriction (CR) or Control diets.Moderate
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