Research
Hormonal
Alternate Day Fasting (ADF) induces greater stress resistance and Sirt1 upregulation in vitro than Continuous Calorie Restriction (CR) or Control diets.
If you want to maximize cellular stress resistance, ADF might be more effective than continuous calorie restriction. This study suggests ADF triggers stronger protective responses in cells.
ModerateQualifiesMEDIUM confidence
Serum collected at the end of the diet period from participants on the ADF regimen induced significant decreases in proliferation, increases in heat shock resistance and increased Sirt1 levels compared to baseline serum, while serum samples from participants on the CR regimen also induced significant increases in Sirt1 levels in addition to significant increases in PGC-1a mRNA expression compared to baseline serum.
Why this rating
In vitro study; comparative data is strong but surrogate endpoints.
Source
In Vitro Cellular Adaptations of Indicators of Longevity in Response to Treatment with Serum Collected from Humans on Calorie Restricted Diets
Joanne Allard et al. · PLoS ONE · 2008
DOI 10.1371/journal.pone.0003211
mechanism_only · n=42Cited 70×
Read the paper DOI resolved against Crossref · corpus check 2026-06-10
More from this paper
- Serum from humans undergoing Alternate Day Fasting (ADF) induces in vitro cellular adaptations associated with longevity, including increased Sirt1 protein, reduced proliferation, and enhanced heat stress resistance.Moderate
- Serum from humans undergoing Continuous Calorie Restriction (CR) increases Sirt1 protein and PGC-1a mRNA levels in vitro, indicating metabolic and mitochondrial adaptations associated with longevity.Moderate
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