Mixed
Using a single muscle biopsy with a long tracer incorporation time (approx. 3 hours) yields reliable myofibrillar protein synthesis rates comparable to the traditional two-biopsy method.
If you are measuring muscle protein synthesis using stable isotopes, you can safely use a single muscle biopsy instead of two, provided you extend the tracer incorporation time to approximately 3 hours. This reduces subject discomfort and risk without compromising the reliability of the results, as long as you use plasma protein for baseline enrichment.
These data demonstrate that the single biopsy approach yields comparable rates of muscle protein synthesis, provided a longer incorporation time is utilized, to that seen with a traditional two biopsy approach.
Why this rating
Randomized controlled validation study with clear statistical comparison (P=0.3) between methods, though small sample size (n=8).
Source
Validation of a single biopsy approach and bolus protein feeding to determine myofibrillar protein synthesis in stable isotope tracer studies in humans
Nicholas A. Burd et al. · Nutrition & Metabolism · 2011
DOI 10.1186/1743-7075-8-15
Related findings · Mixed
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