Research
Energy balance
Methylene blue does not significantly extend median lifespan in male or female mice, but significantly increases maximum lifespan (90th percentile) in females.
Methylene blue did not extend the average lifespan of mice, but it did slightly increase the maximum lifespan of female mice. This suggests a very limited potential for extending the very end of life, but not overall longevity.
ModerateQualifiesLOW confidence
MB did not alter median lifespan of males or females, but did produce a small, statistically significant (6%, P = 0.004) increase in female maximum lifespan.
Why this rating
Statistically significant but small effect (6%) on maximum lifespan only; no median lifespan benefit.
Source
Acarbose, 17‐α‐estradiol, and nordihydroguaiaretic acid extend mouse lifespan preferentially in males
David E. Harrison et al. · Aging Cell · 2013
DOI 10.1111/acel.12170
rctCited 461×
Read the paper DOI resolved against Crossref · corpus check 2026-06-10
More from this paper
- Acarbose extends median lifespan in male mice significantly more than in female mice, despite reducing body weight more in females.Good
- Nordihydroguaiaretic acid (NDGA) extends median lifespan in male mice across multiple doses, but has no effect on female lifespan even when blood levels are matched to males.Good
- 17-alpha-estradiol extends median lifespan in male mice, with effects varying significantly by testing site, but has no effect on female lifespan.Moderate
Related findings · Energy balance
- Achieving a total body weight loss of 10-15% (or >10-15 kg) through Total Diet Replacement (TDR) induces remission of Type 2 Diabetes in individuals with short-duration disease.Strong
- Bariatric surgery is superior to medical management alone for inducing significant long-term weight loss, remission of type 2 diabetes, and reduction in mortality for patients with BMI ≥ 40 or ≥ 35 with comorbidities.Strong
- Achieving type 2 diabetes remission requires significant weight loss (≥15 kg) via major caloric restriction, independent of macronutrient composition.Strong
This is one finding among thousands. Every one is graded and traced to its source, so you can see what the evidence actually supports. Browse the research →