Hormonal
Electrical pulse stimulation (EPS) of primary human myotubes increases the secretion of the myokine PEDF in vitro, even though mRNA expression levels remain unchanged, indicating post-transcriptional regulation.
This finding explains how muscles can rapidly release signaling proteins during exercise without immediately changing their genetic instructions. It highlights the efficiency of the muscular system in adapting to physical stress through pre-stored or rapidly released factors.
Although PEDF secretion from primary human myotubes was significantly enhanced after 8 and 24 h of EPS (Fig. 3E), measurement of PEDF serum level after 60 min cycling revealed a decrease 30 min post exercise (Fig. 3F)... Relative mRNA expression was not changed after 24 h EPS (Fig. 3D).
Why this rating
Strong in vitro validation using ELISA and Western blot across multiple donors, supported by human in vivo data showing the downstream serum effect.
Source
Identification and Validation of Novel Contraction-Regulated Myokines Released from Primary Human Skeletal Muscle Cells
Silja Raschke et al. · PLoS ONE · 2013
DOI 10.1371/journal.pone.0062008
More from this paper
- Skeletal muscle cells secrete 52 novel myokines and 48 additional contraction-regulated myokines, expanding the known human skeletal muscle secretome beyond previously identified proteins like IL-6 and irisin.Strong
- Acute high-intensity cycling exercise (70% VO2max for 60 minutes) causes a transient decrease in serum levels of the myokine PEDF, which normalizes within 120 minutes post-exercise, despite increased local secretion from contracting muscle cells.Good
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